Journal: Pharmaceuticals

Article Title: Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

doi: 10.3390/ph18030345

Figure Lengend Snippet: Figure 5. Anti-inflammatory activity of OpvE. (A) The pretreatment of OpvE was able to protect the LPS-induced reduced cell viability by attenuating the transcription of NF-κB (B), TNF-α (C), MYD88 (D), and the translation of TLR4, NF-κB, p-NF-κB, IL-6, and TNF-α (E). All the values are denoted as means ± SD (n = 3). * p < 0.05, ** p < 0.01 vs. UT group, # p < 0.05, ## p < 0.01 vs. LPS group.

Article Snippet: Antigen Dilution Vendor Catalog No. p-PI3K 1:1000 Cell Signaling Technology(Danvers, MA, USA) 17366 PI3K 1:1000 Cell Signaling Technology 4292 p-AKT 1:1000 BOSTER(Pleasanton, CA, USA) BM4721 AKT 1:1000 BOSTER A00024-2 p-GSK3β 1:1000 BOSTER BM4837 GSK3β 1:10,000 BOSTER A00791-3 TLR4 1:1000 BOSTER AF8208 NF-κB 1:1000 BOSTER AF0246 p-NF-κB 1:1000 Cell Signaling Technology 3033 TNF-α 1:1000 BOSTER BA0131 IL-6 1:1000 BOSTER BA0562-2 NRF2 1:1000 BOSTER A00078-1 COX2 1:1000 BOSTER A00084 HO-1 1:1000 BOSTER PB0050 GPX4 1:1000 BOSTER A02059-1 β-actin 1:5000 BOSTER BM3873 GAPDH 1:5000 BOSTER BM3876 Pharmaceuticals 2025, 18, 345 16 of 17 Table A5.

Techniques: Activity Assay

Journal: Pharmaceuticals

Article Title: Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

doi: 10.3390/ph18030345

Figure Lengend Snippet: Figure 4. The antioxidative capability of OpvE. (A) The free radical-scavenging activity of OpvE and vitamin C was comparatively evaluated with DPPH assay tests. (B) The pretreatment of OpvE was able to protect the H2O2-induced reduced cell viability by improving the GSH (C) and SOD (D) levels and downregulating the MDA levels (E) in HaCaT cells. The western blotting result and the semi- quantitative analysis (F) of the expression of Nrf2, COX2, HO-1, and GPX4 in the aforementioned various HaCaT cell assays. All the values are denoted as means ± SD (n = 3). ** p < 0.01 vs. UT group, # p < 0.05, ## p < 0.01 vs. H2O2 group.

Article Snippet: Antigen Dilution Vendor Catalog No. p-PI3K 1:1000 Cell Signaling Technology(Danvers, MA, USA) 17366 PI3K 1:1000 Cell Signaling Technology 4292 p-AKT 1:1000 BOSTER(Pleasanton, CA, USA) BM4721 AKT 1:1000 BOSTER A00024-2 p-GSK3β 1:1000 BOSTER BM4837 GSK3β 1:10,000 BOSTER A00791-3 TLR4 1:1000 BOSTER AF8208 NF-κB 1:1000 BOSTER AF0246 p-NF-κB 1:1000 Cell Signaling Technology 3033 TNF-α 1:1000 BOSTER BA0131 IL-6 1:1000 BOSTER BA0562-2 NRF2 1:1000 BOSTER A00078-1 COX2 1:1000 BOSTER A00084 HO-1 1:1000 BOSTER PB0050 GPX4 1:1000 BOSTER A02059-1 β-actin 1:5000 BOSTER BM3873 GAPDH 1:5000 BOSTER BM3876 Pharmaceuticals 2025, 18, 345 16 of 17 Table A5.

Techniques: Activity Assay, DPPH Assay, Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway

doi: 10.3389/fphar.2019.01459

Figure Lengend Snippet: Effects of magnolol on NLRP3 inflammasome, caspase-1 and caspase-3 signaling pathway in ALD mice. Magnolol was given to mice for three times, and then alcohol was gavaged. The level of NLRP3 inflammasome, caspase-1 and caspase-3 was detected by western blotting analysis with the compared with the internal control (GAPDH). The data is presented as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: Cox-2 and CYP2E1 Elisa kits were bought from Shanghai Lanpai Biotechnology co. LTD. Antibodies against GAPDH, p-AKT, AKT, Nrf2, HO-1, NLRP3, p-PI3K, and PI3K were purchased from Boster bioengineering co. LTD (Wuhan, China).

Techniques: Western Blot, Control